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This study was conducted to find out the suitable culture conditions on adventitious bud formation from callus of Gladiolus ´Topaz´. Calli were induced from cormel tissue of Gladiolus ´Topaz´ on MS medium supplemented with 10 ㎎·L^(-1) 2,4-D. The calli were subcultured on medium with 0.5 ㎎·L^(-1) NAA, 0.25 ㎎·L^(-1) NAA + 0.25 ㎎·L^(-1) 2,4-D, or 0.5 ㎎·L^(-1) 2,4-D, respectively. The fresh and dry weights of callus were in the order of NAA, NAA+2,4-D, 2,4-D during subculture, but cell numbers were in the order of 2,4-D, NAA+2,4-D, NAA. The cell division activity was higher in callus subcultured on the medium added with 2,4-D than the medium with NAA or NAA+2,4-D. The medium with 2,4-D 0.5 ㎎·L^(-1) was appropriate for subculturing callus, since no organ formation took place. Cell division activity was the highest on the callus cultured for 20 days after transplanting to the new subcultural medium. Adventitious bud formation from the callus was favorable on medium supplemented with lower level of BA (0.01-0.1 ㎎·L^(-1)) or without BA, regardless of auxin types during subculture of the callus. Adventitious bud formation and days required adventitious bud formation were stimulated with the increase of medium volume and NH₄NO₃ concentration.
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